1: Li C, McCrone S, Warrick JW, Andes DR, Hite Z, Volk CF, Rose WE, Beebe DJ.
Under-oil open microfluidic systems for rapid phenotypic antimicrobial
susceptibility testing. Lab Chip. 2023 Apr 12;23(8):2005-2015. doi:
10.1039/d3lc00066d. PMID: 36883560.
2: McMinn PH, Ahmed A, Huttenlocher A, Beebe DJ, Kerr SC. The lymphatic
endothelium-derived follistatin: activin A axis regulates neutrophil motility in
response to Pseudomonas aeruginosa. Integr Biol (Camb). 2023 Apr 11;15:zyad003.
doi: 10.1093/intbio/zyad003. PMID: 36781971.
3: Zhao SG, Sperger JM, Schehr JL, McKay RR, Emamekhoo H, Singh A, Schultz ZD,
Bade RM, Stahlfeld CN, Gilsdorf CS, Hernandez CI, Wolfe SK, Mayberry RD, Krause
HM, Bootsma M, Helzer KT, Rydzewski N, Bakhtiar H, Shi Y, Blitzer G,
Kyriakopoulos CE, Kosoff D, Wei XX, Floberg J, Sethakorn N, Sharifi M, Harari
PM, Huang W, Beltran H, Choueiri TK, Scher HI, Rathkopf DE, Halabi S, Armstrong
AJ, Beebe DJ, Yu M, Sundling KE, Taplin ME, Lang JM. A clinical-grade liquid
biomarker detects neuroendocrine differentiation in prostate cancer. J Clin
Invest. 2022 Nov 1;132(21):e161858. doi: 10.1172/JCI161858. PMID: 36317634;
PMCID: PMC9621140.
4: Fakhraldeen SA, Berry SM, Beebe DJ, Alexander CM. Enhanced Ribonucleoprotein
Immunoprecipitation (RIP) Technique for the Identification of mRNA Species in
Ribonucleoprotein Complexes. Bio Protoc. 2022 Oct 5;12(19):e4526. doi:
10.21769/BioProtoc.4526. PMID: 36313200; PMCID: PMC9548516.
5: Sethakorn N, Heninger E, Breneman MT, Recchia E, Ding AB, Jarrard DF, Hematti
P, Beebe DJ, Kosoff D. Integrated analysis of the tumor microenvironment using a
reconfigurable microfluidic cell culture platform. FASEB J. 2022
Oct;36(10):e22540. doi: 10.1096/fj.202200684RR. PMID: 36083096; PMCID:
PMC9476232.
6: Rodems TS, Heninger E, Stahlfeld CN, Gilsdorf CS, Carlson KN, Kircher MR,
Singh A, Krueger TEG, Beebe DJ, Jarrard DF, McNeel DG, Haffner MC, Lang JM.
Reversible epigenetic alterations regulate class I HLA loss in prostate cancer.
Commun Biol. 2022 Sep 1;5(1):897. doi: 10.1038/s42003-022-03843-6. PMID:
36050516; PMCID: PMC9437063.
7: Simitian G, Virumbrales-Muñoz M, Sánchez-de-Diego C, Beebe DJ, Kosoff D.
Microfluidics in vascular biology research: a critical review for engineers,
biologists, and clinicians. Lab Chip. 2022 Sep 27;22(19):3618-3636. doi:
10.1039/d2lc00352j. PMID: 36047330; PMCID: PMC9530010.
8: Denecke KM, McBain CA, Hermes BG, Teertam SK, Farooqui M, Virumbrales-Muñoz
M, Panackal J, Beebe DJ, Famakin B, Ayuso JM. Microfluidic Model to Evaluate
Astrocyte Activation in Penumbral Region following Ischemic Stroke. Cells. 2022
Jul 31;11(15):2356. doi: 10.3390/cells11152356. PMID: 35954200; PMCID:
PMC9367413.
9: Ayuso JM, Virumbrales-Muñoz M, Lang JM, Beebe DJ. A role for microfluidic
systems in precision medicine. Nat Commun. 2022 Jun 2;13(1):3086. doi:
10.1038/s41467-022-30384-7. PMID: 35654785; PMCID: PMC9163169.
10: Humayun M, Ayuso JM, Park KY, Martorelli Di Genova B, Skala MC, Kerr SC,
Knoll LJ, Beebe DJ. Innate immune cell response to host-parasite interaction in
a human intestinal tissue microphysiological system. Sci Adv. 2022 May
6;8(18):eabm8012. doi: 10.1126/sciadv.abm8012. Epub 2022 May 6. PMID: 35544643;
PMCID: PMC9075809.
11: Schehr JL, Sethakorn N, Schultz ZD, Hernandez CI, Bade RM, Eyzaguirre D,
Singh A, Niles DJ, Henderson L, Warrick JW, Berry SM, Sundling KE, Beebe DJ,
Leal TA, Lang JM. Analytical validation and initial clinical testing of
quantitative microscopic evaluation for PD-L1 and HLA I expression on
circulating tumor cells from patients with non-small cell lung cancer. Biomark
Res. 2022 Apr 25;10(1):26. doi: 10.1186/s40364-022-00370-8. PMID: 35468853;
PMCID: PMC9040226.
12: Shapiro DD, Virumbrales-Muñoz M, Beebe DJ, Abel EJ. Models of Renal Cell
Carcinoma Used to Investigate Molecular Mechanisms and Develop New Therapeutics.
Front Oncol. 2022 Apr 7;12:871252. doi: 10.3389/fonc.2022.871252. PMID:
35463327; PMCID: PMC9022005.
13: Virumbrales-Muñoz M, Ayuso JM, Loken JR, Denecke KM, Rehman S, Skala MC,
Abel EJ, Beebe DJ. Microphysiological model of renal cell carcinoma to inform
anti-angiogenic therapy. Biomaterials. 2022 Apr;283:121454. doi:
10.1016/j.biomaterials.2022.121454. Epub 2022 Mar 11. PMID: 35299086; PMCID:
PMC9254636.
14: Rodems TS, Juang DS, Stahlfeld CN, Gilsdorf CS, Krueger TEG, Heninger E,
Zhao SG, Sperger JM, Beebe DJ, Haffner MC, Lang JM. SEEMLIS: a flexible semi-
automated method for enrichment of methylated DNA from low-input samples. Clin
Epigenetics. 2022 Mar 10;14(1):37. doi: 10.1186/s13148-022-01252-4. PMID:
35272673; PMCID: PMC8908705.
15: Sadangi S, Milosavljevic K, Castro-Perez E, Lares M, Singh M, Altameemi S,
Beebe DJ, Ayuso JM, Setaluri V. Role of the Skin Microenvironment in
Melanomagenesis: Epidermal Keratinocytes and Dermal Fibroblasts Promote BRAF
Oncogene-Induced Senescence Escape in Melanocytes. Cancers (Basel). 2022 Feb
27;14(5):1233. doi: 10.3390/cancers14051233. PMID: 35267541; PMCID: PMC8909265.
16: Vitek RA, Huang W, Geiger PG, Heninger E, Lang JM, Jarrard DF, Beebe DJ,
Johnson BP. Fresh tissue procurement and preparation for multicompartment and
multimodal analysis of the prostate tumor microenvironment. Prostate. 2022
May;82(7):836-849. doi: 10.1002/pros.24326. Epub 2022 Feb 28. PMID: 35226381;
PMCID: PMC9010374.
17: Sethakorn N, Heninger E, S√°nchez-de-Diego C, Ding AB, Yada RC, Kerr SC,
Kosoff D, Beebe DJ, Lang JM. Advancing Treatment of Bone Metastases through
Novel Translational Approaches Targeting the Bone Microenvironment. Cancers
(Basel). 2022 Feb 1;14(3):757. doi: 10.3390/cancers14030757. PMID: 35159026;
PMCID: PMC8833657.
18: Li C, Humayun M, Walker GM, Park KY, Connors B, Feng J, Pellitteri Hahn MC,
Scarlett CO, Li J, Feng Y, Clark RL, Hefti H, Schrope J, Venturelli OS, Beebe
- Under-Oil Autonomously Regulated Oxygen Microenvironments: A Goldilocks
Principle-Based Approach for Microscale Cell Culture. Adv Sci (Weinh). 2022
Apr;9(10):e2104510. doi: 10.1002/advs.202104510. Epub 2022 Feb 4. PMID:
35118834; PMCID: PMC8981459.
19: Fakhraldeen SA, Berry SM, Beebe DJ, Roopra A, Bisbach CM, Spiegelman VS,
Niemi NM, Alexander CM. Enhanced immunoprecipitation techniques for the
identification of RNA-binding protein partners: IGF2BP1 interactions in mammary
epithelial cells. J Biol Chem. 2022 Mar;298(3):101649. doi:
10.1016/j.jbc.2022.101649. Epub 2022 Jan 29. PMID: 35104504; PMCID: PMC8891971.
20: Park KY, Hefti HO, Liu P, Lugo-Cintrón KM, Kerr SC, Beebe DJ. Immune cell
mediated cabozantinib resistance for patients with renal cell carcinoma. Integr
Biol (Camb). 2021 Dec 30;13(11):259-268. doi: 10.1093/intbio/zyab018. PMID:
34931665; PMCID: PMC8730366.
21: Babatunde KA, Ayuso JM, Kerr SC, Huttenlocher A, Beebe DJ. Microfluidic
Systems to Study Neutrophil Forward and Reverse Migration. Front Immunol. 2021
Nov 24;12:781535. doi: 10.3389/fimmu.2021.781535. PMID: 34899746; PMCID:
PMC8653704.
22: Juang DS, Lang JM, Beebe DJ. Volumeless reagent delivery: a liquid handling
method for adding reagents to microscale droplets without increasing volume. Lab
Chip. 2022 Jan 18;22(2):286-295. doi: 10.1039/d1lc00906k. PMID: 34897347; PMCID:
PMC8820037.
23: Lugo-Cintrón KM, Ayuso JM, Humayun M, Gong MM, Kerr SC, Ponik SM, Harari PM,
Virumbrales-Muñoz M, Beebe DJ. Primary head and neck tumour-derived fibroblasts
promote lymphangiogenesis in a lymphatic organotypic co-culture model.
EBioMedicine. 2021 Nov;73:103634. doi: 10.1016/j.ebiom.2021.103634. Epub 2021
Oct 18. PMID: 34673450; PMCID: PMC8528684.
24: van Steen ACI, Kempers L, Schoppmeyer R, Blokker M, Beebe DJ, Nolte MA, van
Buul JD. Transendothelial migration induces differential migration dynamics of
leukocytes in tissue matrix. J Cell Sci. 2021 Nov 1;134(21):jcs258690. doi:
10.1242/jcs.258690. Epub 2021 Nov 4. PMID: 34622930; PMCID: PMC8627551.
25: Li C, Hurley A, Hu W, Warrick JW, Lozano GL, Ayuso JM, Pan W, Handelsman J,
Beebe DJ. Social motility of biofilm-like microcolonies in a gliding bacterium.
Nat Commun. 2021 Sep 29;12(1):5700. doi: 10.1038/s41467-021-25408-7. PMID:
34588437; PMCID: PMC8481357.
26: Hu Y, Manasrah BK, McGregor SM, Lera RF, Norman RX, Tucker JB, Scribano CM,
Yan RE, Humayun M, Wisinski KB, Tevaarwerk AJ, O’Regan RM, Wilke LG, Weaver BA,
Beebe DJ, Jin N, Burkard ME. Paclitaxel Induces Micronucleation and Activates
Pro-Inflammatory cGAS-STING Signaling in Triple-Negative Breast Cancer. Mol
Cancer Ther. 2021 Dec;20(12):2553-2567. doi: 10.1158/1535-7163.MCT-21-0195. Epub
2021 Sep 28. PMID: 34583980; PMCID: PMC8643310.
27: Heninger E, Kosoff D, Rodems TS, Sethakorn N, Singh A, Gungurthi H, Carlson
KN, Yang B, Gilsdorf C, Pasch CA, Deming DA, Ellis L, Beebe DJ, Jarrard DF, Lang
- Live cell molecular analysis of primary prostate cancer organoids identifies
persistent androgen receptor signaling. Med Oncol. 2021 Sep 28;38(11):135. doi:
10.1007/s12032-021-01582-y. PMID: 34581895; PMCID: PMC8478748.
28: Juang DS, Juang TD, Dudley DM, Newman CM, Accola MA, Rehrauer WM, Friedrich
TC, O’Connor DH, Beebe DJ. Oil immersed lossless total analysis system for
integrated RNA extraction and detection of SARS-CoV-2. Nat Commun. 2021 Jul
14;12(1):4317. doi: 10.1038/s41467-021-24463-4. PMID: 34262053; PMCID:
PMC8280165.
29: Hind LE, Giese MA, Schoen TJ, Beebe DJ, Keller N, Huttenlocher A. Immune
Cell Paracrine Signaling Drives the Neutrophil Response to <i>A. fumigatus</i>
in an Infection-on-a-Chip Model. Cell Mol Bioeng. 2020 Oct 13;14(2):133-145.
doi: 10.1007/s12195-020-00655-8. PMID: 33868496; PMCID: PMC8010091.
30: Yu J, Piazza A, Sparks S, Hind LE, Niles DJ, Ingram PN, Huang W, Ricke WA,
Jarrard DF, Huttenlocher A, Basu H, Beebe DJ. A reconfigurable microscale assay
enables insights into cancer-associated fibroblast modulation of immune cell
recruitment. Integr Biol (Camb). 2021 Apr 20;13(4):87-97. doi:
10.1093/intbio/zyab004. PMID: 33822934; PMCID: PMC8793920.
31: Johnson BP, Vitek RA, Morgan MM, Fink DM, Beames TG, Geiger PG, Beebe DJ,
Lipinski RJ. A Microphysiological Approach to Evaluate Effectors of
Intercellular Hedgehog Signaling in Development. Front Cell Dev Biol. 2021 Feb
9;9:621442. doi: 10.3389/fcell.2021.621442. PMID: 33634122; PMCID: PMC7900501.
32: Bade RM, Schehr JL, Emamekhoo H, Gibbs BK, Rodems TS, Mannino MC, Desotelle
JA, Heninger E, Stahlfeld CN, Sperger JM, Singh A, Wolfe SK, Niles DJ, Arafat W,
Steinharter JA, Jason Abel E, Beebe DJ, Wei XX, McKay RR, Choueri TK, Lang JM.
Development and initial clinical testing of a multiplexed circulating tumor cell
assay in patients with clear cell renal cell carcinoma. Mol Oncol. 2021
Sep;15(9):2330-2344. doi: 10.1002/1878-0261.12931. Epub 2021 Mar 3. PMID:
33604999; PMCID: PMC8410529.
33: Ayuso JM, Rehman S, Virumbrales-Munoz M, McMinn PH, Geiger P, Fitzgerald C,
Heaster T, Skala MC, Beebe DJ. Microfluidic tumor-on-a-chip model to evaluate
the role of tumor environmental stress on NK cell exhaustion. Sci Adv. 2021 Feb
17;7(8):eabc2331. doi: 10.1126/sciadv.abc2331. PMID: 33597234; PMCID:
PMC7888951.
34: Humayun M, Ayuso JM, Brenneke RA, Virumbrales-Muñoz M, Lugo-Cintrón K, Kerr
S, Ponik SM, Beebe DJ. Elucidating cancer-vascular paracrine signaling using a
human organotypic breast cancer cell extravasation model. Biomaterials. 2021
Mar;270:120640. doi: 10.1016/j.biomaterials.2020.120640. Epub 2021 Feb 6. PMID:
33592387; PMCID: PMC9703973.
35: Ayuso JM, Sadangi S, Lares M, Rehman S, Humayun M, Denecke KM, Skala MC,
Beebe DJ, Setaluri V. Microfluidic model with air-walls reveals fibroblasts and
keratinocytes modulate melanoma cell phenotype, migration, and metabolism. Lab
Chip. 2021 Mar 21;21(6):1139-1149. doi: 10.1039/d0lc00988a. Epub 2021 Feb 3.
PMID: 33533390; PMCID: PMC7990711.
36: Ayuso JM, Park KY, Virumbrales-Muñoz M, Beebe DJ. Toward improved <i>in
vitro</i> models of human cancer. APL Bioeng. 2021 Jan 21;5(1):010902. doi:
10.1063/5.0026857. PMID: 33532672; PMCID: PMC7822630.
37: Ayuso JM, Rehman S, Farooqui M, Virumbrales-Muñoz M, Setaluri V, Skala MC,
Beebe DJ. Microfluidic Tumor-on-a-Chip Model to Study Tumor Metabolic
Vulnerability. Int J Mol Sci. 2020 Nov 28;21(23):9075. doi:
10.3390/ijms21239075. PMID: 33260673; PMCID: PMC7730115.
38: Virumbrales-Muñoz M, Chen J, Ayuso J, Lee M, Abel EJ, Beebe DJ. Organotypic
primary blood vessel models of clear cell renal cell carcinoma for single-
patient clinical trials. Lab Chip. 2020 Nov 24;20(23):4420-4432. doi:
10.1039/d0lc00252f. PMID: 33103699; PMCID: PMC8743028.
39: Heaster TM, Humayun M, Yu J, Beebe DJ, Skala MC. Autofluorescence Imaging of
3D Tumor-Macrophage Microscale Cultures Resolves Spatial and Temporal Dynamics
of Macrophage Metabolism. Cancer Res. 2020 Dec 1;80(23):5408-5423. doi:
10.1158/0008-5472.CAN-20-0831. Epub 2020 Oct 22. PMID: 33093167; PMCID:
PMC7718391.
40: Kerr SC, Morgan MM, Gillette AA, Livingston MK, Lugo-Cintron KM, Favreau PF,
Florek L, Johnson BP, Lang JM, Skala MC, Beebe DJ. A bioengineered organotypic
prostate model for the study of tumor microenvironment-induced immune cell
activation. Integr Biol (Camb). 2020 Oct 16;12(10):250-262. doi:
10.1093/intbio/zyaa020. PMID: 33034643; PMCID: PMC7569006.
41: Virumbrales-Muñoz M, Ayuso JM, Gong MM, Humayun M, Livingston MK, Lugo-
Cintrón KM, McMinn P, Álvarez-García YR, Beebe DJ. Microfluidic lumen-based
systems for advancing tubular organ modeling. Chem Soc Rev. 2020 Sep
1;49(17):6402-6442. doi: 10.1039/d0cs00705f. PMID: 32760967; PMCID: PMC7521761.
42: Kaushik G, Gupta K, Harms V, Torr E, Evans J, Johnson HJ, Soref C, Acevedo-
Acevedo S, Antosiewicz-Bourget J, Mamott D, Uhl P, Johnson BP, Palecek SP, Beebe
DJ, Thomson JA, Daly WT, Murphy WL. Engineered Perineural Vascular Plexus for
Modeling Developmental Toxicity. Adv Healthc Mater. 2020 Aug;9(16):e2000825.
doi: 10.1002/adhm.202000825. Epub 2020 Jul 1. PMID: 32613760; PMCID: PMC8016604.
43: McMinn P, Guckenberger DJ, Beebe DJ. Induced Pluripotent Stem Cells on a
Chip: A Self-Contained, Accessible, Pipette-less iPSC Culturing and
Differentiation Kit. SLAS Technol. 2021 Feb;26(1):80-91. doi:
10.1177/2472630320921173. Epub 2020 Jun 19. PMID: 32552316.
44: Li C, Hite Z, Warrick JW, Li J, Geller SH, Trantow VG, McClean MN, Beebe DJ.
Under oil open-channel microfluidics empowered by exclusive liquid repellency.
Sci Adv. 2020 Apr 17;6(16):eaay9919. doi: 10.1126/sciadv.aay9919. PMID:
32494607; PMCID: PMC7164933.
45: Lugo-Cintrón KM, Gong MM, Ayuso JM, Tomko LA, Beebe DJ, Virumbrales-Muñoz M,
Ponik SM. Breast Fibroblasts and ECM Components Modulate Breast Cancer Cell
Migration Through the Secretion of MMPs in a 3D Microfluidic Co-Culture Model.
Cancers (Basel). 2020 May 6;12(5):1173. doi: 10.3390/cancers12051173. PMID:
32384738; PMCID: PMC7281408.
46: Lugo-Cintrón KM, Ayuso JM, White BR, Harari PM, Ponik SM, Beebe DJ, Gong MM,
Virumbrales-Muñoz M. Matrix density drives 3D organotypic lymphatic vessel
activation in a microfluidic model of the breast tumor microenvironment. Lab
Chip. 2020 May 7;20(9):1586-1600. doi: 10.1039/d0lc00099j. Epub 2020 Apr 16.
PMID: 32297896; PMCID: PMC7330815.
47: Morgan MM, Schuler LA, Ciciliano JC, Johnson BP, Alarid ET, Beebe DJ.
Modeling chemical effects on breast cancer: the importance of the
microenvironment in vitro. Integr Biol (Camb). 2020 Mar 6;12(2):21-33. doi:
10.1093/intbio/zyaa002. PMID: 32118264; PMCID: PMC7060306.
48: Whitt J, Hong WS, Telange RR, Lin CP, Bibb J, Beebe DJ, Chen H, Jaskula-
Sztul R. Non-toxic fragment of botulinum neurotoxin type A and monomethyl
auristatin E conjugate for targeted therapy for neuroendocrine tumors. Cancer
Gene Ther. 2020 Dec;27(12):898-909. doi: 10.1038/s41417-020-0167-x. Epub 2020
Feb 7. PMID: 32029905.
49: Tokar JJ, Stahlfeld CN, Sperger JM, Niles DJ, Beebe DJ, Lang JM, Warrick JW.
Pairing Microwell Arrays with an Affordable, Semiautomated Single-Cell Aspirator
for the Interrogation of Circulating Tumor Cell Heterogeneity. SLAS Technol.
2020 Apr;25(2):162-176. doi: 10.1177/2472630319898146. Epub 2020 Jan 26. PMID:
31983266; PMCID: PMC8879417.
50: Livingston MK, Morgan MM, Daly WT, Murphy WL, Johnson BP, Beebe DJ,
Virumbrales-Muñoz M. Evaluation of PEG-based hydrogel influence on estrogen
receptor driven responses in MCF7 breast cancer cells. ACS Biomater Sci Eng.
2019;5(11):6089-6098. doi: 10.1021/acsbiomaterials.9b00480. PMID: 31942444;
PMCID: PMC6961958.
51: Virumbrales-Muñoz M, Ayuso JM, Lacueva A, Randelovic T, Livingston MK, Beebe
DJ, Oliv√°n S, Pereboom D, Doblare M, Fern√°ndez L, Ochoa I. Author Correction:
Enabling cell recovery from 3D cell culture microfluidic devices for tumour
microenvironment biomarker profiling. Sci Rep. 2020 Jan 15;10(1):757. doi:
10.1038/s41598-020-57830-0. Erratum for: Sci Rep. 2019 Apr 17;9(1):6199. PMID:
31937889; PMCID: PMC6960145.
52: Ayuso JM, Gong MM, Skala MC, Harari PM, Beebe DJ. Human Tumor-Lymphatic
Microfluidic Model Reveals Differential Conditioning of Lymphatic Vessels by
Breast Cancer Cells. Adv Healthc Mater. 2020 Feb;9(3):e1900925. doi:
10.1002/adhm.201900925. Epub 2020 Jan 1. PMID: 31894641; PMCID: PMC7004876.
53: Virumbrales-Muñoz M, Livingston MK, Farooqui M, Skala MC, Beebe DJ, Ayuso
- Development of a Microfluidic Array to Study Drug Response in Breast Cancer.
Molecules. 2019 Nov 30;24(23):4385. doi: 10.3390/molecules24234385. PMID:
31801265; PMCID: PMC6930663.
54: McMinn PH, Hind LE, Huttenlocher A, Beebe DJ. Neutrophil trafficking on-a-
chip: an in vitro, organotypic model for investigating neutrophil priming,
extravasation, and migration with spatiotemporal control. Lab Chip. 2019 Nov
7;19(21):3697-3705. doi: 10.1039/c9lc00562e. Epub 2019 Oct 2. PMID: 31576879;
PMCID: PMC7045365.
55: Ayuso JM, Virumbrales-Munoz M, McMinn PH, Rehman S, Gomez I, Karim MR,
Trusttchel R, Wisinski KB, Beebe DJ, Skala MC. Tumor-on-a-chip: a microfluidic
model to study cell response to environmental gradients. Lab Chip. 2019 Oct
9;19(20):3461-3471. doi: 10.1039/c9lc00270g. PMID: 31506657; PMCID: PMC6785375.
56: Ayuso JM, Vitek R, Swick AD, Skala MC, Wisinski KB, Kimple RJ, Lambert PF,
Beebe DJ. Effects of culture method on response to EGFR therapy in head and neck
squamous cell carcinoma cells. Sci Rep. 2019 Aug 28;9(1):12480. doi:
10.1038/s41598-019-48764-3. PMID: 31462653; PMCID: PMC6713778.
57: Yu J, Berthier E, Craig A, de Groot TE, Sparks S, Ingram PN, Jarrard DF,
Huang W, Beebe DJ, Theberge AB. Reconfigurable open microfluidics for studying
the spatiotemporal dynamics of paracrine signalling. Nat Biomed Eng. 2019
Oct;3(10):830-841. doi: 10.1038/s41551-019-0421-4. Epub 2019 Aug 19. PMID:
31427781; PMCID: PMC7543914.
58: Juang DS, Berry SM, Li C, Lang JM, Beebe DJ. Centrifugation-Assisted
Immiscible Fluid Filtration for Dual-Bioanalyte Extraction. Anal Chem. 2019 Sep
17;91(18):11848-11855. doi: 10.1021/acs.analchem.9b02572. Epub 2019 Aug 26.
PMID: 31411020; PMCID: PMC7521759.
59: Li C, Niles DJ, Juang DS, Lang JM, Beebe DJ. Automated System for Small-
Population Single-Particle Processing Enabled by Exclusive Liquid Repellency.
SLAS Technol. 2019 Dec;24(6):535-542. doi: 10.1177/2472630319853219. Epub 2019
Jun 10. PMID: 31180792; PMCID: PMC7521760.
60: Gong MM, Lugo-Cintron KM, White BR, Kerr SC, Harari PM, Beebe DJ. Human
organotypic lymphatic vessel model elucidates microenvironment-dependent
signaling and barrier function. Biomaterials. 2019 Sep;214:119225. doi:
10.1016/j.biomaterials.2019.119225. Epub 2019 May 25. PMID: 31154151; PMCID:
PMC7032699.
61: Seenivasan R, Warrick JW, Rodriguez CI, Mattison W, Beebe DJ, Setaluri V,
Gunasekaran S. Integrating Electrochemical Immunosensing and Cell Adhesion
Technologies for Cancer Cell Detection and Enumeration. Electrochim Acta. 2018
Oct 1;286:205-211. doi: 10.1016/j.electacta.2018.08.005. Epub 2018 Aug 4. PMID:
31130739; PMCID: PMC6530932.
62: Regier MC, Olszewski E, Carter CC, Aitchison JD, Kaushansky A, Davis J,
Berthier E, Beebe DJ, Stevens KR. Spatial presentation of biological molecules
to cells by localized diffusive transfer. Lab Chip. 2019 Jun
11;19(12):2114-2126. doi: 10.1039/c9lc00122k. PMID: 31111131; PMCID: PMC6755031.
63: Regier MC, Tokar JJ, Warrick JW, Pabon L, Berthier E, Beebe DJ, Stevens KR.
User-defined morphogen patterning for directing human cell fate stratification.
Sci Rep. 2019 Apr 23;9(1):6433. doi: 10.1038/s41598-019-42874-8. PMID: 31015521;
PMCID: PMC6478938.
64: Morgan MM, Arendt LM, Alarid ET, Beebe DJ, Johnson BP. Mammary adipose
stromal cells derived from obese women reduce sensitivity to the aromatase
inhibitor anastrazole in an organotypic breast model. FASEB J. 2019
Jul;33(7):8623-8633. doi: 10.1096/fj.201802347RRR. Epub 2019 Apr 19. PMID:
31002529; PMCID: PMC6593876.
65: Virumbrales-Muñoz M, Ayuso JM, Lacueva A, Randelovic T, Livingston MK, Beebe
DJ, Oliv√°n S, Pereboom D, Doblare M, Fern√°ndez L, Ochoa I. Enabling cell
recovery from 3D cell culture microfluidic devices for tumour microenvironment
biomarker profiling. Sci Rep. 2019 Apr 17;9(1):6199. doi:
10.1038/s41598-019-42529-8. Erratum in: Sci Rep. 2020 Jan 15;10(1):757. PMID:
30996291; PMCID: PMC6470149.
66: Jiménez-Torres JA, Virumbrales-Muñoz M, Sung KE, Lee MH, Abel EJ, Beebe DJ.
Patient-specific organotypic blood vessels as an in vitro model for anti-
angiogenic drug response testing in renal cell carcinoma. EBioMedicine. 2019
Apr;42:408-419. doi: 10.1016/j.ebiom.2019.03.026. Epub 2019 Mar 20. PMID:
30902740; PMCID: PMC6491391.
67: Ayuso JM, Truttschel R, Gong MM, Humayun M, Virumbrales-Munoz M, Vitek R,
Felder M, Gillies SD, Sondel P, Wisinski KB, Patankar M, Beebe DJ, Skala MC.
Evaluating natural killer cell cytotoxicity against solid tumors using a
microfluidic model. Oncoimmunology. 2018 Dec 12;8(3):1553477. doi:
10.1080/2162402X.2018.1553477. PMID: 30723584; PMCID: PMC6350694.
68: Ayuso JM, Gillette A, Lugo-Cintrón K, Acevedo-Acevedo S, Gomez I, Morgan M,
Heaster T, Wisinski KB, Palecek SP, Skala MC, Beebe DJ. Organotypic microfluidic
breast cancer model reveals starvation-induced spatial-temporal metabolic
adaptations. EBioMedicine. 2018 Nov;37:144-157. doi:
10.1016/j.ebiom.2018.10.046. Epub 2018 Oct 26. PMID: 30482722; PMCID:
PMC6284542.
69: Pezzi HM, Guckenberger DJ, Schehr JL, Rothbauer J, Stahlfeld C, Singh A,
Horn S, Schultz ZD, Bade RM, Sperger JM, Berry SM, Lang JM, Beebe DJ. Versatile
exclusion-based sample preparation platform for integrated rare cell isolation
and analyte extraction. Lab Chip. 2018 Nov 6;18(22):3446-3458. doi:
10.1039/c8lc00620b. PMID: 30334061; PMCID: PMC6402328.
70: Álvarez-García YR, Ramos-Cruz KP, Agostini-Infanzón RJ, Stallcop LE, Beebe
DJ, Warrick JW, Domenech M. Open multi-culture platform for simple and flexible
study of multi-cell type interactions. Lab Chip. 2018 Oct 9;18(20):3184-3195.
doi: 10.1039/c8lc00560e. PMID: 30204194; PMCID: PMC8815088.
71: Hind LE, Ingram PN, Beebe DJ, Huttenlocher A. Interaction with an
endothelial lumen increases neutrophil lifetime and motility in response to <i>P
aeruginosa</i>. Blood. 2018 Oct 25;132(17):1818-1828. doi:
10.1182/blood-2018-05-848465. Epub 2018 Aug 24. PMID: 30143504; PMCID:
PMC6202912.
72: Kosoff D, Yu J, Suresh V, Beebe DJ, Lang JM. Surface topography and
hydrophilicity regulate macrophage phenotype in milled microfluidic systems. Lab
Chip. 2018 Sep 26;18(19):3011-3017. doi: 10.1039/c8lc00431e. PMID: 30131982;
PMCID: PMC6178814.
73: Li C, Yu J, Paine P, Juang DS, Berry SM, Beebe DJ. Double-exclusive liquid
repellency (double-ELR): an enabling technology for rare phenotype analysis. Lab
Chip. 2018 Sep 11;18(18):2710-2719. doi: 10.1039/c8lc00584b. PMID: 30069559;
PMCID: PMC6402335.
74: Morgan MM, Livingston MK, Warrick JW, Stanek EM, Alarid ET, Beebe DJ,
Johnson BP. Mammary fibroblasts reduce apoptosis and speed estrogen-induced
hyperplasia in an organotypic MCF7-derived duct model. Sci Rep. 2018 May
8;8(1):7139. doi: 10.1038/s41598-018-25461-1. PMID: 29740030; PMCID: PMC5940820.
75: Li C, Yu J, Schehr J, Berry SM, Leal TA, Lang JM, Beebe DJ. Exclusive Liquid
Repellency: An Open Multi-Liquid-Phase Technology for Rare Cell Culture and
Single-Cell Processing. ACS Appl Mater Interfaces. 2018 May
23;10(20):17065-17070. doi: 10.1021/acsami.8b03627. Epub 2018 May 8. PMID:
29738227; PMCID: PMC9703972.
76: Pezzi HM, Niles DJ, Schehr JL, Beebe DJ, Lang JM. Integration of Magnetic
Bead-Based Cell Selection into Complex Isolations. ACS Omega. 2018 Apr
30;3(4):3908-3917. doi: 10.1021/acsomega.7b01427. Epub 2018 Apr 6. PMID:
29732449; PMCID: PMC5928489.
77: Johnson BP, Vitek RA, Geiger PG, Huang W, Jarrard DF, Lang JM, Beebe DJ.
Vital ex vivo tissue labeling and pathology-guided micropunching to characterize
cellular heterogeneity in the tissue microenvironment. Biotechniques. 2018 Jan
1;64(1):13-19. doi: 10.2144/000114626. PMID: 29384072; PMCID: PMC5814138.
78: Ingram PN, Hind LE, Jiminez-Torres JA, Huttenlocher A, Beebe DJ. An
Accessible Organotypic Microvessel Model Using iPSC-Derived Endothelium. Adv
Healthc Mater. 2018 Jan;7(2):10.1002/adhm.201700497. doi:
10.1002/adhm.201700497. Epub 2017 Aug 7. PMID: 29364596; PMCID: PMC6402823.
79: Poenitzsch Strong AM, Berry SM, Beebe DJ, Li JL, Spiegelman VS. miFAST: A
novel and rapid microRNA target capture method. Mol Carcinog. 2018
Apr;57(4):559-566. doi: 10.1002/mc.22780. Epub 2018 Feb 12. PMID: 29350431;
PMCID: PMC8858646.
80: Stallcop LE, Álvarez-García YR, Reyes-Ramos AM, Ramos-Cruz KP, Morgan MM,
Shi Y, Li L, Beebe DJ, Domenech M, Warrick JW. Razor-printed sticker
microdevices for cell-based applications. Lab Chip. 2018 Jan 30;18(3):451-462.
doi: 10.1039/c7lc00724h. PMID: 29318250; PMCID: PMC5821501.
81: Barkal LJ, Procknow CL, Álvarez-García YR, Niu M, Jiménez-Torres JA,
Brockman-Schneider RA, Gern JE, Denlinger LC, Theberge AB, Keller NP, Berthier
E, Beebe DJ. Microbial volatile communication in human organotypic lung models.
Nat Commun. 2017 Nov 24;8(1):1770. doi: 10.1038/s41467-017-01985-4. PMID:
29176665; PMCID: PMC5701243.
82: Pezzi HM, Berry SM, Beebe DJ, Striker R. RNA-mediated TILDA for improved
cell capacity and enhanced detection of multiply-spliced HIV RNA. Integr Biol
(Camb). 2017 Nov 13;9(11):876-884. doi: 10.1039/c7ib00112f. PMID: 29098230;
PMCID: PMC5733636.
83: Spurgeon ME, den Boon JA, Horswill M, Barthakur S, Forouzan O, Rader JS,
Beebe DJ, Roopra A, Ahlquist P, Lambert PF. Human papillomavirus oncogenes
reprogram the cervical cancer microenvironment independently of and
synergistically with estrogen. Proc Natl Acad Sci U S A. 2017 Oct
24;114(43):E9076-E9085. doi: 10.1073/pnas.1712018114. Epub 2017 Oct 9. PMID:
29073104; PMCID: PMC5664542.
84: Barkal LJ, Berthier E, Theberge AB, Keller NP, Beebe DJ. Multikingdom
microscale models. PLoS Pathog. 2017 Aug 24;13(8):e1006424. doi:
10.1371/journal.ppat.1006424. PMID: 28837694; PMCID: PMC5570211.
85: Lam J, Marklein RA, Jimenez-Torres JA, Beebe DJ, Bauer SR, Sung KE.
Adaptation of a Simple Microfluidic Platform for High-Dimensional Quantitative
Morphological Analysis of Human Mesenchymal Stromal Cells on Polystyrene-Based
Substrates. SLAS Technol. 2017 Dec;22(6):646-661. doi: 10.1177/2472630317726050.
Epub 2017 Aug 21. PMID: 28825968; PMCID: PMC7222604.
86: Powell D, Tauzin S, Hind LE, Deng Q, Beebe DJ, Huttenlocher A. Chemokine
Signaling and the Regulation of Bidirectional Leukocyte Migration in
Interstitial Tissues. Cell Rep. 2017 May 23;19(8):1572-1585. doi:
10.1016/j.celrep.2017.04.078. PMID: 28538177; PMCID: PMC5505660.
87: Tokar JJ, Warrick JW, Guckenberger DJ, Sperger JM, Lang JM, Ferguson JS,
Beebe DJ. Interrogating Bronchoalveolar Lavage Samples via Exclusion-Based
Analyte Extraction. SLAS Technol. 2017 Jun;22(3):348-357. doi:
10.1177/2472630317696780. Epub 2017 Mar 15. PMID: 28298147; PMCID: PMC5603149.
88: Regier MC, Montanez-Sauri SI, Schwartz MP, Murphy WL, Beebe DJ, Sung KE. The
Influence of Biomaterials on Cytokine Production in 3D Cultures.
Biomacromolecules. 2017 Mar 13;18(3):709-718. doi: 10.1021/acs.biomac.6b01469.
Epub 2017 Feb 16. PMID: 28157290; PMCID: PMC5672812.
89: Johnson CJ, Cabezas-Olcoz J, Kernien JF, Wang SX, Beebe DJ, Huttenlocher A,
Ansari H, Nett JE. The Extracellular Matrix of Candida albicans Biofilms Impairs
Formation of Neutrophil Extracellular Traps. PLoS Pathog. 2016 Sep
13;12(9):e1005884. doi: 10.1371/journal.ppat.1005884. PMID: 27622514; PMCID:
PMC5021349.
90: Guckenberger DJ, Pezzi HM, Regier MC, Berry SM, Fawcett K, Barrett K, Beebe
- Magnetic System for Automated Manipulation of Paramagnetic Particles. Anal
Chem. 2016 Oct 18;88(20):9902-9907. doi: 10.1021/acs.analchem.6b02257. Epub 2016
Oct 3. PMID: 27598856; PMCID: PMC5378678.
91: Jiménez-Torres JA, Beebe DJ, Sung KE. A Microfluidic Method to Mimic Luminal
Structures in the Tumor Microenvironment. Methods Mol Biol. 2016;1458:59-69.
doi: 10.1007/978-1-4939-3801-8_5. PMID: 27581014.
92: Schehr JL, Schultz ZD, Warrick JW, Guckenberger DJ, Pezzi HM, Sperger JM,
Heninger E, Saeed A, Leal T, Mattox K, Traynor AM, Campbell TC, Berry SM, Beebe
DJ, Lang JM. High Specificity in Circulating Tumor Cell Identification Is
Required for Accurate Evaluation of Programmed Death-Ligand 1. PLoS One. 2016
Jul 26;11(7):e0159397. doi: 10.1371/journal.pone.0159397. PMID: 27459545; PMCID:
PMC4961410.
93: Regier MC, Maccoux LJ, Weinberger EM, Regehr KJ, Berry SM, Beebe DJ, Alarid
- Transitions from mono- to co- to tri-culture uniquely affect gene expression
in breast cancer, stromal, and immune compartments. Biomed Microdevices. 2016
Aug;18(4):70. doi: 10.1007/s10544-016-0083-x. PMID: 27432323; PMCID: PMC5076020.
94: Sperger JM, Strotman LN, Welsh A, Casavant BP, Chalmers Z, Horn S, Heninger
E, Thiede SM, Tokar J, Gibbs BK, Guckenberger DJ, Carmichael L, Dehm SM,
Stephens PJ, Beebe DJ, Berry SM, Lang JM. Integrated Analysis of Multiple
Biomarkers from Circulating Tumor Cells Enabled by Exclusion-Based Analyte
Isolation. Clin Cancer Res. 2017 Feb;23(3):746-756. doi:
10.1158/1078-0432.CCR-16-1021. Epub 2016 Jul 11. PMID: 27401243; PMCID:
PMC5226928.
95: Berry SM, Pezzi HM, LaVanway AJ, Guckenberger DJ, Anderson MA, Beebe DJ.
AirJump: Using Interfaces to Instantly Perform Simultaneous Extractions. ACS
Appl Mater Interfaces. 2016 Jun 22;8(24):15040-5. doi: 10.1021/acsami.6b02555.
Epub 2016 Jun 10. PMID: 27249333; PMCID: PMC5058634.
96: Morgan MM, Johnson BP, Livingston MK, Schuler LA, Alarid ET, Sung KE, Beebe
- Personalized in vitro cancer models to predict therapeutic response:
Challenges and a framework for improvement. Pharmacol Ther. 2016 Sep;165:79-92.
doi: 10.1016/j.pharmthera.2016.05.007. Epub 2016 May 21. PMID: 27218886; PMCID:
PMC5439438.
97: Zasadil LM, Britigan EM, Ryan SD, Kaur C, Guckenberger DJ, Beebe DJ, Moser
AR, Weaver BA. High rates of chromosome missegregation suppress tumor
progression but do not inhibit tumor initiation. Mol Biol Cell. 2016 Jul
1;27(13):1981-9. doi: 10.1091/mbc.E15-10-0747. Epub 2016 May 4. PMID: 27146113;
PMCID: PMC4927272.
98: Regier MC, Alarid ET, Beebe DJ. Progress towards understanding heterotypic
interactions in multi-culture models of breast cancer. Integr Biol (Camb). 2016
Jun 13;8(6):684-92. doi: 10.1039/c6ib00001k. Epub 2016 Apr 21. PMID: 27097801;
PMCID: PMC4993016.
99: Barkal LJ, Walsh NM, Botts MR, Beebe DJ, Hull CM. Leveraging a high
resolution microfluidic assay reveals insights into pathogenic fungal spore
germination. Integr Biol (Camb). 2016 May 16;8(5):603-15. doi:
10.1039/c6ib00012f. Epub 2016 Mar 30. PMID: 27026574; PMCID: PMC4868663.
100: Zanotelli MR, Ardalani H, Zhang J, Hou Z, Nguyen EH, Swanson S, Nguyen BK,
Bolin J, Elwell A, Bischel LL, Xie AW, Stewart R, Beebe DJ, Thomson JA, Schwartz
MP, Murphy WL. Stable engineered vascular networks from human induced
pluripotent stem cell-derived endothelial cells cultured in synthetic hydrogels.
Acta Biomater. 2016 Apr 15;35:32-41. doi: 10.1016/j.actbio.2016.03.001. Epub
2016 Mar 2. PMID: 26945632; PMCID: PMC4829480.
101: Barkal LJ, Theberge AB, Guo CJ, Spraker J, Rappert L, Berthier J, Brakke
KA, Wang CCC, Beebe DJ, Keller NP, Berthier E. Microbial metabolomics in open
microscale platforms. Nat Commun. 2016 Feb 4;7:10610. doi: 10.1038/ncomms10610.
PMID: 26842393; PMCID: PMC4742997.
102: Moussavi-Harami SF, Mladinich KM, Sackmann EK, Shelef MA, Starnes TW,
Guckenberger DJ, Huttenlocher A, Beebe DJ. Microfluidic device for simultaneous
analysis of neutrophil extracellular traps and production of reactive oxygen
species. Integr Biol (Camb). 2016 Feb;8(2):243-52. doi: 10.1039/c5ib00225g. Epub
2016 Jan 25. PMID: 26805445; PMCID: PMC4776335.
103: de Groot TE, Veserat KS, Berthier E, Beebe DJ, Theberge AB. Surface-tension
driven open microfluidic platform for hanging droplet culture. Lab Chip. 2016
Jan 21;16(2):334-44. doi: 10.1039/c5lc01353d. PMID: 26660268; PMCID: PMC4712910.
104: Berry SM, Pezzi HM, Williams ED, Loeb JM, Guckenberger DJ, Lavanway AJ,
Puchalski AA, Kityo CM, Mugyenyi PN, Graziano FM, Beebe DJ. Using Exclusion-
Based Sample Preparation (ESP) to Reduce Viral Load Assay Cost. PLoS One. 2015
Dec 2;10(12):e0143631. doi: 10.1371/journal.pone.0143631. PMID: 26630135; PMCID:
PMC4667969.
105: Jiménez-Torres JA, Peery SL, Sung KE, Beebe DJ. LumeNEXT: A Practical
Method to Pattern Luminal Structures in ECM Gels. Adv Healthc Mater. 2016 Jan
21;5(2):198-204. doi: 10.1002/adhm.201500608. Epub 2015 Nov 26. PMID: 26610188;
PMCID: PMC4776323.
106: Hong WS, Pezzi HM, Schuster AR, Berry SM, Sung KE, Beebe DJ. Development of
a Highly Sensitive Cell-Based Assay for Detecting Botulinum Neurotoxin Type A
through Neural Culture Media Optimization. J Biomol Screen. 2016
Jan;21(1):65-73. doi: 10.1177/1087057115608103. Epub 2015 Sep 29. PMID:
26420788; PMCID: PMC6988902.
107: Pak C, Callander NS, Young EW, Titz B, Kim K, Saha S, Chng K, Asimakopoulos
F, Beebe DJ, Miyamoto S. MicroC(3): an ex vivo microfluidic cis-coculture assay
to test chemosensitivity and resistance of patient multiple myeloma cells.
Integr Biol (Camb). 2015 Jun;7(6):643-54. doi: 10.1039/c5ib00071h. Epub 2015 May
- PMID: 25998180; PMCID: PMC4476551.
108: Moussavi-Harami SF, Wisinski KB, Beebe DJ. Circulating Tumor Cells in
Metastatic Breast Cancer: A Prognostic and Predictive Marker. J Patient Cent Res
Rev. 2014 Spring;1(2):85-92. doi: 10.17294/2330-0698.1017. PMID: 25914894;
PMCID: PMC4405902.
109: Guckenberger DJ, de Groot TE, Wan AM, Beebe DJ, Young EW. Micromilling: a
method for ultra-rapid prototyping of plastic microfluidic devices. Lab Chip.
2015 Jun 7;15(11):2364-78. doi: 10.1039/c5lc00234f. PMID: 25906246; PMCID:
PMC4439323.
110: Moussavi-Harami SF, Pezzi HM, Huttenlocher A, Beebe DJ. Simple microfluidic
device for studying chemotaxis in response to dual gradients. Biomed
Microdevices. 2015;17(3):9955. doi: 10.1007/s10544-015-9955-8. PMID: 25893484;
PMCID: PMC4768479.
111: Theberge AB, Yu J, Young EW, Ricke WA, Bushman W, Beebe DJ. Microfluidic
multiculture assay to analyze biomolecular signaling in angiogenesis. Anal Chem.
2015 Mar 17;87(6):3239-46. doi: 10.1021/ac503700f. Epub 2015 Feb 26. PMID:
25719435; PMCID: PMC4405103.
112: Bischel LL, Beebe DJ, Sung KE. Microfluidic model of ductal carcinoma in
situ with 3D, organotypic structure. BMC Cancer. 2015 Jan 21;15:12. doi:
10.1186/s12885-015-1007-5. PMID: 25605670; PMCID: PMC4305264.
113: Wan J, Zhu F, Zasadil LM, Yu J, Wang L, Johnson A, Berthier E, Beebe DJ,
Audhya A, Weaver BA. A Golgi-localized pool of the mitotic checkpoint component
Mad1 controls integrin secretion and cell migration. Curr Biol. 2014 Nov
17;24(22):2687-92. doi: 10.1016/j.cub.2014.09.052. Epub 2014 Oct 23. PMID:
25447996; PMCID: PMC4254593.
114: Guckenberger DJ, Berthier E, Beebe DJ. High-density self-contained
microfluidic KOALA kits for use by everyone. J Lab Autom. 2015 Apr;20(2):146-53.
doi: 10.1177/2211068214560609. Epub 2014 Nov 25. PMID: 25424385; PMCID:
PMC4707655.
115: Montanez-Sauri SI, Beebe DJ, Sung KE. Microscale screening systems for 3D
cellular microenvironments: platforms, advances, and challenges. Cell Mol Life
Sci. 2015 Jan;72(2):237-49. doi: 10.1007/s00018-014-1738-5. Epub 2014 Oct 2.
PMID: 25274061; PMCID: PMC4629475.
116: Powers GL, Hammer KD, Domenech M, Frantskevich K, Malinowski RL, Bushman W,
Beebe DJ, Marker PC. Phosphodiesterase 4D inhibitors limit prostate cancer
growth potential. Mol Cancer Res. 2015 Jan;13(1):149-60. doi:
10.1158/1541-7786.MCR-14-0110. Epub 2014 Aug 22. PMID: 25149359; PMCID:
PMC4312503.
117: Carney CM, Muszynski JL, Strotman LN, Lewis SR, O’Connell RL, Beebe DJ,
Theberge AB, Jorgensen JS. Cellular microenvironment dictates androgen
production by murine fetal Leydig cells in primary culture. Biol Reprod. 2014
Oct;91(4):85. doi: 10.1095/biolreprod.114.118570. Epub 2014 Aug 20. PMID:
25143354; PMCID: PMC4435030.
118: Bischel LL, Sung KE, Jiménez-Torres JA, Mader B, Keely PJ, Beebe DJ. The
importance of being a lumen. FASEB J. 2014 Nov;28(11):4583-90. doi:
10.1096/fj.13-243733. Epub 2014 Jul 30. PMID: 25077562; PMCID: PMC4200326.
119: Guckenberger DJ, Berthier E, Young EW, Beebe DJ. Fluorescence-based
assessment of plasma-induced hydrophilicity in microfluidic devices via Nile Red
adsorption and depletion. Anal Chem. 2014 Aug 5;86(15):7258-63. doi:
10.1021/ac501259n. Epub 2014 Jul 17. PMID: 25032783; PMCID: PMC4144722.
120: Sung KE, Beebe DJ. Microfluidic 3D models of cancer. Adv Drug Deliv Rev.
2014 Dec 15;79-80:68-78. doi: 10.1016/j.addr.2014.07.002. Epub 2014 Jul 10.
PMID: 25017040; PMCID: PMC4258433.
121: Berthier E, Beebe DJ. Gradient generation platforms: new directions for an
established microfluidic technology. Lab Chip. 2014 Sep 7;14(17):3241-7. doi:
10.1039/c4lc00448e. PMID: 25008971; PMCID: PMC4134926.
122: Casavant BP, Guckenberger DJ, Beebe DJ, Berry SM. Efficient sample
preparation from complex biological samples using a sliding lid for immobilized
droplet extractions. Anal Chem. 2014 Jul 1;86(13):6355-62. doi:
10.1021/ac500574t. Epub 2014 Jun 13. PMID: 24927449; PMCID: PMC4079323.
123: Bischel LL, Casavant BP, Young PA, Eliceiri KW, Basu HS, Beebe DJ. A
microfluidic coculture and multiphoton FAD analysis assay provides insight into
the influence of the bone microenvironment on prostate cancer cells. Integr Biol
(Camb). 2014 Jun;6(6):627-635. doi: 10.1039/c3ib40240a. PMID: 24791272; PMCID:
PMC4077588.
124: Sackmann EK, Berthier E, Schwantes EA, Fichtinger PS, Evans MD, Dziadzio
LL, Huttenlocher A, Mathur SK, Beebe DJ. Characterizing asthma from a drop of
blood using neutrophil chemotaxis. Proc Natl Acad Sci U S A. 2014 Apr
22;111(16):5813-8. doi: 10.1073/pnas.1324043111. Epub 2014 Apr 7. PMID:
24711384; PMCID: PMC4000787.
125: Sackmann EK, Fulton AL, Beebe DJ. The present and future role of
microfluidics in biomedical research. Nature. 2014 Mar 13;507(7491):181-9. doi:
10.1038/nature13118. PMID: 24622198.
126: Berry SM, LaVanway AJ, Pezzi HM, Guckenberger DJ, Anderson MA, Loeb JM,
Beebe DJ. HIV viral RNA extraction in wax immiscible filtration assisted by
surface tension (IFAST) devices. J Mol Diagn. 2014 May;16(3):297-304. doi:
10.1016/j.jmoldx.2014.01.004. Epub 2014 Mar 6. PMID: 24613822; PMCID:
PMC4715211.
127: Shelef MA, Sokolove J, Lahey LJ, Wagner CA, Sackmann EK, Warner TF, Wang Y,
Beebe DJ, Robinson WH, Huttenlocher A. Peptidylarginine deiminase 4 contributes
to tumor necrosis factor α-induced inflammatory arthritis. Arthritis Rheumatol.
2014 Jun;66(6):1482-91. doi: 10.1002/art.38393. PMID: 24497204; PMCID:
PMC4148484.
128: Berry SM, Singh C, Lang JD, Strotman LN, Alarid ET, Beebe DJ. Streamlining
gene expression analysis: integration of co-culture and mRNA purification.
Integr Biol (Camb). 2014 Feb;6(2):224-31. doi: 10.1039/c3ib40136g. PMID:
24413730; PMCID: PMC3956049.
129: Guckenberger DJ, Thomas PC, Rothbauer J, LaVanway AJ, Anderson M, Gilson D,
Fawcett K, Berto T, Barrett K, Beebe DJ, Berry SM. A Combined Fabrication and
Instrumentation Platform for Sample Preparation. J Lab Autom. 2014
Jun;19(3):267-74. doi: 10.1177/2211068213518312. Epub 2014 Jan 8. PMID:
24401822.
130: Schwartz MP, Rogers RE, Singh SP, Lee JY, Loveland SG, Koepsel JT, Witze
ES, Montanez-Sauri SI, Sung KE, Tokuda EY, Sharma Y, Everhart LM, Nguyen EH,
Zaman MH, Beebe DJ, Ahn NG, Murphy WL, Anseth KS. A quantitative comparison of
human HT-1080 fibrosarcoma cells and primary human dermal fibroblasts identifies
a 3D migration mechanism with properties unique to the transformed phenotype.
PLoS One. 2013 Dec 3;8(12):e81689. doi: 10.1371/journal.pone.0081689. PMID:
24349113; PMCID: PMC3857815.
131: Fan ZH, Beebe DJ. Lab on a chip and circulating tumor cells. Lab Chip. 2014
Jan 7;14(1):12-3. doi: 10.1039/c3lc90125d. PMID: 24247213.
132: Beebe DJ, Lang JM. Editorial for “methods for the isolation and analysis of
rare cells”. Methods. 2013 Dec 1;64(2):101. doi: 10.1016/j.ymeth.2013.10.017.
PMID: 24238070.
133: Berry SM, Chin EN, Jackson SS, Strotman LN, Goel M, Thompson NE, Alexander
CM, Miyamoto S, Burgess RR, Beebe DJ. Weak protein-protein interactions revealed
by immiscible filtration assisted by surface tension. Anal Biochem. 2014 Feb
15;447:133-40. doi: 10.1016/j.ab.2013.10.038. Epub 2013 Nov 9. PMID: 24215910;
PMCID: PMC3897128.
134: Casavant BP, Strotman LN, Tokar JJ, Thiede SM, Traynor AM, Ferguson JS,
Lang JM, Beebe DJ. Paired diagnostic and pharmacodynamic analysis of rare non-
small cell lung cancer cells enabled by the VerIFAST platform. Lab Chip. 2014
Jan 7;14(1):99-105. doi: 10.1039/c3lc50912e. PMID: 24158597; PMCID: PMC3897162.
135: Howard AL, Pezzi HM, Beebe DJ, Berry SM. Exclusion-Based Capture and
Enumeration of CD4+ T Cells from Whole Blood for Low-Resource Settings. J Lab
Autom. 2014 Jun;19(3):313-21. doi: 10.1177/2211068213509248. Epub 2013 Oct 18.
PMID: 24142472; PMCID: PMC4300285.
136: Sung KE, Su X, Berthier E, Pehlke C, Friedl A, Beebe DJ. Understanding the
impact of 2D and 3D fibroblast cultures on in vitro breast cancer models. PLoS
One. 2013 Oct 4;8(10):e76373. doi: 10.1371/journal.pone.0076373. PMID: 24124550;
PMCID: PMC3790689.
137: Strotman L, O’Connell R, Casavant BP, Berry SM, Sperger JM, Lang JM, Beebe
- Selective nucleic acid removal via exclusion (SNARE): capturing mRNA and DNA
from a single sample. Anal Chem. 2013 Oct 15;85(20):9764-70. doi:
10.1021/ac402162r. Epub 2013 Sep 26. PMID: 24016179; PMCID: PMC3897163.
138: Thomas PC, Strotman LN, Theberge AB, Berthier E, O’Connell R, Loeb JM,
Berry SM, Beebe DJ. Nucleic acid sample preparation using spontaneous biphasic
plug flow. Anal Chem. 2013 Sep 17;85(18):8641-6. doi: 10.1021/ac4012914. Epub
2013 Sep 4. PMID: 23941230; PMCID: PMC3858960.
139: Beebe DJ, Ingber DE, den Toonder J. Organs on Chips 2013. Lab Chip. 2013
Sep 21;13(18):3447-8. doi: 10.1039/c3lc90080k. PMID: 23918086.
140: Casavant BP, Mosher R, Warrick JW, Maccoux LJ, Berry SM, Becker JT, Chen V,
Lang JM, McNeel DG, Beebe DJ. A negative selection methodology using a
microfluidic platform for the isolation and enumeration of circulating tumor
cells. Methods. 2013 Dec 1;64(2):137-43. doi: 10.1016/j.ymeth.2013.05.027. Epub
2013 Jun 24. PMID: 23806645; PMCID: PMC3858973.
141: Moussavi-Harami SF, Annis DS, Ma W, Berry SM, Coughlin EE, Strotman LN,
Maurer LM, Westphall MS, Coon JJ, Mosher DF, Beebe DJ. Characterization of
molecules binding to the 70K N-terminal region of fibronectin by IFAST
purification coupled with mass spectrometry. J Proteome Res. 2013 Jul
5;12(7):3393-404. doi: 10.1021/pr400225p. Epub 2013 Jun 21. PMID: 23750785;
PMCID: PMC3832424.
142: Casavant BP, Berthier E, Theberge AB, Berthier J, Montanez-Sauri SI,
Bischel LL, Brakke K, Hedman CJ, Bushman W, Keller NP, Beebe DJ. Suspended
microfluidics. Proc Natl Acad Sci U S A. 2013 Jun 18;110(25):10111-6. doi:
10.1073/pnas.1302566110. Epub 2013 May 31. PMID: 23729815; PMCID: PMC3690848.
143: Berthier E, Lim FY, Deng Q, Guo CJ, Kontoyiannis DP, Wang CC, Rindy J,
Beebe DJ, Huttenlocher A, Keller NP. Low-volume toolbox for the discovery of
immunosuppressive fungal secondary metabolites. PLoS Pathog. 2013;9(4):e1003289.
doi: 10.1371/journal.ppat.1003289. Epub 2013 Apr 11. PMID: 23592999; PMCID:
PMC3623715.
144: Hong WS, Young EW, Tepp WH, Johnson EA, Beebe DJ. A microscale neuron and
Schwann cell coculture model for increasing detection sensitivity of botulinum
neurotoxin type A. Toxicol Sci. 2013 Jul;134(1):64-72. doi:
10.1093/toxsci/kft082. Epub 2013 Apr 5. PMID: 23564642.
145: Lang JD, Berry SM, Powers GL, Beebe DJ, Alarid ET. Hormonally responsive
breast cancer cells in a microfluidic co-culture model as a sensor of
microenvironmental activity. Integr Biol (Camb). 2013 May;5(5):807-16. doi:
10.1039/c3ib20265h. PMID: 23559098; PMCID: PMC3648339.
146: Bischel LL, Mader BR, Green JM, Huttenlocher A, Beebe DJ. Zebrafish
Entrapment By Restriction Array (ZEBRA) device: a low-cost, agarose-free
zebrafish mounting technique for automated imaging. Lab Chip. 2013 May
7;13(9):1732-6. doi: 10.1039/c3lc50099c. PMID: 23503983; PMCID: PMC4446983.
147: Warrick JW, Young EW, Schmuck EG, Saupe KW, Beebe DJ. High-content adhesion
assay to address limited cell samples. Integr Biol (Camb). 2013 Apr;5(4):720-7.
doi: 10.1039/c3ib20224k. Epub 2013 Feb 21. PMID: 23426645; PMCID: PMC3832292.
148: Montanez-Sauri SI, Sung KE, Berthier E, Beebe DJ. Enabling screening in 3D
microenvironments: probing matrix and stromal effects on the morphology and
proliferation of T47D breast carcinoma cells. Integr Biol (Camb). 2013
Mar;5(3):631-40. doi: 10.1039/c3ib20225a. PMID: 23340769; PMCID: PMC3613432.
149: Su X, Theberge AB, January CT, Beebe DJ. Effect of microculture on cell
metabolism and biochemistry: do cells get stressed in microchannels? Anal Chem.
2013 Feb 5;85(3):1562-70. doi: 10.1021/ac3027228. Epub 2013 Jan 17. PMID:
23327437; PMCID: PMC3565071.
150: Young EW, Berthier E, Beebe DJ. Assessment of enhanced autofluorescence and
impact on cell microscopy for microfabricated thermoplastic devices. Anal Chem.
2013 Jan 2;85(1):44-9. doi: 10.1021/ac3034773. Epub 2012 Dec 18. PMID: 23249264;
PMCID: PMC4017339.
151: Berthier E, Guckenberger DJ, Cavnar P, Huttenlocher A, Keller NP, Beebe DJ.
Kit-On-A-Lid-Assays for accessible self-contained cell assays. Lab Chip. 2013
Feb 7;13(3):424-31. doi: 10.1039/c2lc41019b. PMID: 23229806; PMCID: PMC3562598.
152: Casavant BP, Guckenberger DJ, Berry SM, Tokar JT, Lang JM, Beebe DJ. The
VerIFAST: an integrated method for cell isolation and
extracellular/intracellular staining. Lab Chip. 2013 Feb 7;13(3):391-6. doi:
10.1039/c2lc41136a. PMID: 23223939.
153: Bischel LL, Young EW, Mader BR, Beebe DJ. Tubeless microfluidic
angiogenesis assay with three-dimensional endothelial-lined microvessels.
Biomaterials. 2013 Feb;34(5):1471-7. doi: 10.1016/j.biomaterials.2012.11.005.
Epub 2012 Nov 26. PMID: 23191982; PMCID: PMC3529167.
154: Su G, Sung KE, Beebe DJ, Friedl A. Functional screen of paracrine signals
in breast carcinoma fibroblasts. PLoS One. 2012;7(10):e46685. doi:
10.1371/journal.pone.0046685. Epub 2012 Oct 8. Erratum in: PLoS One. 2013;8(4).
doi:10.1371/annotation/c5015b96-51d1-4421-8b48-d2478b947959. PMID: 23056402;
PMCID: PMC3466317.
155: Berry SM, Regehr KJ, Casavant BP, Beebe DJ. Automated operation of
immiscible filtration assisted by surface tension (IFAST) arrays for streamlined
analyte isolation. J Lab Autom. 2013 Jun;18(3):206-11. doi:
10.1177/2211068212462023. Epub 2012 Sep 26. PMID: 23015519; PMCID: PMC3633642.
156: Sackmann EK, Berthier E, Young EW, Shelef MA, Wernimont SA, Huttenlocher A,
Beebe DJ. Microfluidic kit-on-a-lid: a versatile platform for neutrophil
chemotaxis assays. Blood. 2012 Oct 4;120(14):e45-53. doi:
10.1182/blood-2012-03-416453. Epub 2012 Aug 22. PMID: 22915642; PMCID:
PMC3466974.
157: Strotman LN, Lin G, Berry SM, Johnson EA, Beebe DJ. Facile and rapid DNA
extraction and purification from food matrices using IFAST (immiscible
filtration assisted by surface tension). Analyst. 2012 Sep 7;137(17):4023-8.
doi: 10.1039/c2an35506j. Epub 2012 Jul 20. PMID: 22814365.
158: Lang JM, Casavant BP, Beebe DJ. Circulating tumor cells: getting more from
less. Sci Transl Med. 2012 Jul 4;4(141):141ps13. doi:
10.1126/scitranslmed.3004261. PMID: 22764205.
159: Cavnar PJ, Mogen K, Berthier E, Beebe DJ, Huttenlocher A. The actin
regulatory protein HS1 interacts with Arp2/3 and mediates efficient neutrophil
chemotaxis. J Biol Chem. 2012 Jul 20;287(30):25466-77. doi:
10.1074/jbc.M112.364562. Epub 2012 Jun 7. PMID: 22679023; PMCID: PMC3408136.
160: Berry SM, Maccoux LJ, Beebe DJ. Streamlining immunoassays with immiscible
filtrations assisted by surface tension. Anal Chem. 2012 Jul 3;84(13):5518-23.
doi: 10.1021/ac300085m. Epub 2012 Jun 19. PMID: 22632629.
161: Guckenberger DJ, Berthier E, Young EW, Beebe DJ. Induced hydrophobic
recovery of oxygen plasma-treated surfaces. Lab Chip. 2012 Jul 7;12(13):2317-21.
doi: 10.1039/c2lc21052e. Epub 2012 May 16. PMID: 22592853; PMCID: PMC4018413.
162: Resto PJ, Berthier E, Beebe DJ, Williams JC. An inertia enhanced passive
pumping mechanism for fluid flow in microfluidic devices. Lab Chip. 2012 Jun
21;12(12):2221-8. doi: 10.1039/c2lc20858j. Epub 2012 Mar 23. PMID: 22441561.
163: Goel S, Chin EN, Fakhraldeen SA, Berry SM, Beebe DJ, Alexander CM. Both
LRP5 and LRP6 receptors are required to respond to physiological Wnt ligands in
mammary epithelial cells and fibroblasts. J Biol Chem. 2012 May
11;287(20):16454-66. doi: 10.1074/jbc.M112.362137. Epub 2012 Mar 20. PMID:
22433869; PMCID: PMC3351289.
164: Bischel LL, Lee SH, Beebe DJ. A practical method for patterning lumens
through ECM hydrogels via viscous finger patterning. J Lab Autom. 2012
Apr;17(2):96-103. doi: 10.1177/2211068211426694. Epub 2012 Jan 24. PMID:
22357560; PMCID: PMC3397721.
165: Young EW, Pak C, Kahl BS, Yang DT, Callander NS, Miyamoto S, Beebe DJ.
Microscale functional cytomics for studying hematologic cancers. Blood. 2012 Mar
8;119(10):e76-85. doi: 10.1182/blood-2011-10-384347. Epub 2012 Jan 18. PMID:
22262772; PMCID: PMC3311264.
166: Domenech M, Bjerregaard R, Bushman W, Beebe DJ. Hedgehog signaling in
myofibroblasts directly promotes prostate tumor cell growth. Integr Biol (Camb).
2012 Feb;4(2):142-52. doi: 10.1039/c1ib00104c. Epub 2012 Jan 11. PMID: 22234342;
PMCID: PMC3335396.
167: Lee AG, Beebe DJ, Palecek SP. Quantification of kinase activity in cell
lysates via photopatterned macroporous poly(ethylene glycol) hydrogel arrays in
microfluidic channels. Biomed Microdevices. 2012 Apr;14(2):247-57. doi:
10.1007/s10544-011-9602-y. PMID: 22069079; PMCID: PMC3299890.
168: Berry SM, Strotman LN, Kueck JD, Alarid ET, Beebe DJ. Purification of cell
subpopulations via immiscible filtration assisted by surface tension (IFAST).
Biomed Microdevices. 2011 Dec;13(6):1033-42. doi: 10.1007/s10544-011-9573-z.
PMID: 21796389; PMCID: PMC3314424.
169: Montanez-Sauri SI, Sung KE, Puccinelli JP, Pehlke C, Beebe DJ. Automation
of three-dimensional cell culture in arrayed microfluidic devices. J Lab Autom.
2011 Jun;16(3):171-85. doi: 10.1016/j.jala.2011.02.003. Epub 2011 May 16. PMID:
21609700; PMCID: PMC3104941.
170: Kshitiz, Kim DH, Beebe DJ, Levchenko A. Micro- and nanoengineering for stem
cell biology: the promise with a caution. Trends Biotechnol. 2011
Aug;29(8):399-408. doi: 10.1016/j.tibtech.2011.03.006. Epub 2011 May 5. PMID:
21549437; PMCID: PMC3726268.
171: Berthier E, Warrick J, Casavant B, Beebe DJ. Pipette-friendly laminar flow
patterning for cell-based assays. Lab Chip. 2011 Jun 21;11(12):2060-5. doi:
10.1039/c0lc00539h. Epub 2011 Apr 26. PMID: 21523269; PMCID: PMC3401607.
172: Cavnar PJ, Berthier E, Beebe DJ, Huttenlocher A. Hax1 regulates neutrophil
adhesion and motility through RhoA. J Cell Biol. 2011 May 2;193(3):465-73. doi:
10.1083/jcb.201010143. Epub 2011 Apr 25. PMID: 21518791; PMCID: PMC3087009.
173: Berry SM, Alarid ET, Beebe DJ. One-step purification of nucleic acid for
gene expression analysis via Immiscible Filtration Assisted by Surface Tension
(IFAST). Lab Chip. 2011 May 21;11(10):1747-53. doi: 10.1039/c1lc00004g. Epub
2011 Mar 21. PMID: 21423999; PMCID: PMC3244820.
174: Lopez-Garcia MD, Beebe DJ, Crone WC. Young’s modulus of collagen at slow
displacement rates. Biomed Mater Eng. 2010;20(6):361-9. doi:
10.3233/BME-2010-0649. PMID: 21263182.
175: Young EW, Berthier E, Guckenberger DJ, Sackmann E, Lamers C, Meyvantsson I,
Huttenlocher A, Beebe DJ. Rapid prototyping of arrayed microfluidic systems in
polystyrene for cell-based assays. Anal Chem. 2011 Feb 15;83(4):1408-17. doi:
10.1021/ac102897h. Epub 2011 Jan 24. PMID: 21261280; PMCID: PMC3052265.
176: Sung KE, Yang N, Pehlke C, Keely PJ, Eliceiri KW, Friedl A, Beebe DJ.
Transition to invasion in breast cancer: a microfluidic in vitro model enables
examination of spatial and temporal effects. Integr Biol (Camb). 2011
Apr;3(4):439-50. doi: 10.1039/c0ib00063a. Epub 2010 Dec 7. PMID: 21135965;
PMCID: PMC3094750.
177: Su X, Young EW, Underkofler HA, Kamp TJ, January CT, Beebe DJ. Microfluidic
cell culture and its application in high-throughput drug screening:
cardiotoxicity assay for hERG channels. J Biomol Screen. 2011 Jan;16(1):101-11.
doi: 10.1177/1087057110386218. Epub 2010 Dec 3. PMID: 21131594; PMCID:
PMC3052261.
178: Lee AG, Arena CP, Beebe DJ, Palecek SP. Development of macroporous
poly(ethylene glycol) hydrogel arrays within microfluidic channels.
Biomacromolecules. 2010 Dec 13;11(12):3316-24. doi: 10.1021/bm100792y. Epub 2010
Oct 28. PMID: 21028794; PMCID: PMC3006031.
179: Beebe DJ, Huttenlocher A. Introduction to the mechanisms of directed cell
migration themed issue. Integr Biol (Camb). 2010 Nov;2(11-12):559-60. doi:
10.1039/c0ib90017f. Epub 2010 Oct 19. PMID: 20957298.
180: Paguirigan AL, Puccinelli JP, Su X, Beebe DJ. Expanding the available
assays: adapting and validating In-Cell Westerns in microfluidic devices for
cell-based assays. Assay Drug Dev Technol. 2010 Oct;8(5):591-601. doi:
10.1089/adt.2010.0274. Epub 2010 Jul 26. PMID: 20658945; PMCID: PMC2957247.
181: Frisk ML, Lin G, Johnson EA, Beebe DJ. Synaptotagmin II peptide-bead
conjugate for botulinum toxin enrichment and detection in microchannels. Biosens
Bioelectron. 2011 Jan 15;26(5):1929-35. doi: 10.1016/j.bios.2010.06.035. Epub
2010 Jun 30. PMID: 20650627.
182: Ju J, Warrick J, Beebe DJ. A Cell Programmable Assay (CPA) chip. Lab Chip.
2010 Aug 21;10(16):2071-6. doi: 10.1039/c005103a. Epub 2010 Jul 8. PMID:
20614082.
183: Bauer M, Su G, Beebe DJ, Friedl A. 3D microchannel co-culture: method and
biological validation. Integr Biol (Camb). 2010 Aug;2(7-8):371-8. doi:
10.1039/c0ib00001a. Epub 2010 Jun 24. PMID: 20577680; PMCID: PMC3025353.
184: Lopez-Garcia MD, Beebe DJ, Crone WC. Mechanical interactions of mouse
mammary gland cells with collagen in a three-dimensional construct. Ann Biomed
Eng. 2010 Aug;38(8):2485-98. doi: 10.1007/s10439-010-0015-5. Epub 2010 Apr 22.
PMID: 20411334.
185: Puccinelli JP, Su X, Beebe DJ. Automated high-throughput microchannel
assays for cell biology: Operational optimization and characterization. JALA
Charlottesv Va. 2010 Feb 1;15(1):25-32. doi: 10.1016/j.jala.2009.10.002. PMID:
20209121; PMCID: PMC2830798.
186: Young EW, Beebe DJ. Fundamentals of microfluidic cell culture in controlled
microenvironments. Chem Soc Rev. 2010 Mar;39(3):1036-48. doi: 10.1039/b909900j.
Epub 2010 Feb 1. PMID: 20179823; PMCID: PMC2967183.
187: Paguirigan AL, Beebe DJ. From the cellular perspective: exploring
differences in the cellular baseline in macroscale and microfluidic cultures.
Integr Biol (Camb). 2009 Feb;1(2):182-95. doi: 10.1039/b814565b. Epub 2009 Jan
- PMID: 20023802; PMCID: PMC3095018.
188: Domenech M, Yu H, Warrick J, Badders NM, Meyvantsson I, Alexander CM, Beebe
- Cellular observations enabled by microculture: paracrine signaling and
population demographics. Integr Biol (Camb). 2009 Mar;1(3):267-74. doi:
10.1039/b823059e. PMID: 20011455; PMCID: PMC2791414.
189: Regehr KJ, Domenech M, Koepsel JT, Carver KC, Ellison-Zelski SJ, Murphy WL,
Schuler LA, Alarid ET, Beebe DJ. Biological implications of
polydimethylsiloxane-based microfluidic cell culture. Lab Chip. 2009 Aug
7;9(15):2132-9. doi: 10.1039/b903043c. Epub 2009 Jun 4. PMID: 19606288; PMCID:
PMC2792742.
190: Sung KE, Su G, Pehlke C, Trier SM, Eliceiri KW, Keely PJ, Friedl A, Beebe
- Control of 3-dimensional collagen matrix polymerization for reproducible
human mammary fibroblast cell culture in microfluidic devices. Biomaterials.
2009 Sep;30(27):4833-41. doi: 10.1016/j.biomaterials.2009.05.043. Epub 2009 Jun
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191: Kim D, Lokuta MA, Huttenlocher A, Beebe DJ. Selective and tunable gradient
device for cell culture and chemotaxis study. Lab Chip. 2009 Jun
21;9(12):1797-800. doi: 10.1039/b901613a. Epub 2009 Mar 26. PMID: 19495465;
PMCID: PMC2804468.
192: Mohanty SK, Warrick J, Gorski J, Beebe DJ. An accessible micro-capillary
electrophoresis device using surface-tension-driven flow. Electrophoresis. 2009
May;30(9):1470-81. doi: 10.1002/elps.200800595. PMID: 19425002; PMCID:
PMC2842211.
193: Frisk ML, Tepp WH, Johnson EA, Beebe DJ. Self-assembled peptide monolayers
as a toxin sensing mechanism within arrayed microchannels. Anal Chem. 2009 Apr
1;81(7):2760-7. doi: 10.1021/ac802707u. PMID: 19253949; PMCID: PMC2760437.
194: Khnouf R, Beebe DJ, Fan ZH. Cell-free protein expression in a microchannel
array with passive pumping. Lab Chip. 2009 Jan 7;9(1):56-61. doi:
10.1039/b808034h. Epub 2008 Oct 22. PMID: 19209336.
195: Zhu Y, Warrick JW, Haubert K, Beebe DJ, Yin J. Infection on a chip: a
microscale platform for simple and sensitive cell-based virus assays. Biomed
Microdevices. 2009 Jun;11(3):565-70. doi: 10.1007/s10544-008-9263-7. PMID:
19142734; PMCID: PMC2869488.
196: Warrick JW, Murphy WL, Beebe DJ. Screening the cellular microenvironment: a
role for microfluidics. IEEE Rev Biomed Eng. 2008;1(1):75-93. doi:
10.1109/RBME.2008.2008241. Epub 2008 Nov 5. PMID: 20190880; PMCID: PMC2829441.
197: Kwon GH, Park JY, Kim JY, Frisk ML, Beebe DJ, Lee SH. Biomimetic soft
multifunctional miniature aquabots. Small. 2008 Dec;4(12):2148-53. doi:
10.1002/smll.200800315. PMID: 18989854.
198: Frisk ML, Berthier E, Tepp WH, Johnson EA, Beebe DJ. Bead-based
microfluidic toxin sensor integrating evaporative signal amplification. Lab
Chip. 2008 Nov;8(11):1793-800. doi: 10.1039/b811075a. Epub 2008 Oct 1. PMID:
18941677.
199: Abhyankar VV, Toepke MW, Cortesio CL, Lokuta MA, Huttenlocher A, Beebe DJ.
A platform for assessing chemotactic migration within a spatiotemporally defined
3D microenvironment. Lab Chip. 2008 Sep;8(9):1507-15. doi: 10.1039/b803533d.
Epub 2008 Jul 16. PMID: 18818806; PMCID: PMC2804469.
200: Paguirigan AL, Beebe DJ. Microfluidics meet cell biology: bridging the gap
by validation and application of microscale techniques for cell biological
assays. Bioessays. 2008 Sep;30(9):811-21. doi: 10.1002/bies.20804. PMID:
18693260; PMCID: PMC2814162.
201: Meyvantsson I, Beebe DJ. Cell culture models in microfluidic systems. Annu
Rev Anal Chem (Palo Alto Calif). 2008;1:423-49. doi:
10.1146/annurev.anchem.1.031207.113042. PMID: 20636085.
202: Berthier E, Warrick J, Yu H, Beebe DJ. Managing evaporation for more robust
microscale assays. Part 2. Characterization of convection and diffusion for cell
biology. Lab Chip. 2008 Jun;8(6):860-4. doi: 10.1039/b717423c. Epub 2008 Apr 8.
PMID: 18497902; PMCID: PMC2453239.
203: Berthier E, Warrick J, Yu H, Beebe DJ. Managing evaporation for more robust
microscale assays. Part 1. Volume loss in high throughput assays. Lab Chip. 2008
Jun;8(6):852-9. doi: 10.1039/b717422e. Epub 2008 Apr 8. PMID: 18497901; PMCID:
PMC2453240.
204: Jongpaiboonkit L, King WJ, Lyons GE, Paguirigan AL, Warrick JW, Beebe DJ,
Murphy WL. An adaptable hydrogel array format for 3-dimensional cell culture and
analysis. Biomaterials. 2008 Aug;29(23):3346-56. doi:
10.1016/j.biomaterials.2008.04.040. Epub 2008 May 16. PMID: 18486205; PMCID:
PMC2490817.
205: Lopez-Garcia MD, Monson RL, Haubert K, Wheeler MB, Beebe DJ. Sperm motion
in a microfluidic fertilization device. Biomed Microdevices. 2008
Oct;10(5):709-18. doi: 10.1007/s10544-008-9182-7. PMID: 18454318.
206: Meyvantsson I, Warrick JW, Hayes S, Skoien A, Beebe DJ. Automated cell
culture in high density tubeless microfluidic device arrays. Lab Chip. 2008
May;8(5):717-24. doi: 10.1039/b715375a. Epub 2008 Mar 20. PMID: 18432341.
207: Beebe DJ. Microscale bioengineering inspired by nature: from widgets to
cell biology. Annu Int Conf IEEE Eng Med Biol Soc. 2007;2007:1. doi:
10.1109/IEMBS.2007.4352206. PMID: 18001872.
208: Berthier E, Beebe DJ. Flow rate analysis of a surface tension driven
passive micropump. Lab Chip. 2007 Nov;7(11):1475-8. doi: 10.1039/b707637a. Epub
2007 Jul 30. PMID: 17960274.
209: Toepke MW, Abhyankar VV, Beebe DJ. Microfluidic logic gates and timers. Lab
Chip. 2007 Nov;7(11):1449-53. doi: 10.1039/b708764k. Epub 2007 Sep 7. PMID:
17960270.
210: Paguirigan AL, Beebe DJ. Protocol for the fabrication of enzymatically
crosslinked gelatin microchannels for microfluidic cell culture. Nat Protoc.
2007;2(7):1782-8. doi: 10.1038/nprot.2007.256. PMID: 17641645.
211: Yu H, Alexander CM, Beebe DJ. Understanding microchannel culture:
parameters involved in soluble factor signaling. Lab Chip. 2007 Jun;7(6):726-30.
doi: 10.1039/b618793e. Epub 2007 Apr 19. PMID: 17538714.
212: Wheeler MB, Walters EM, Beebe DJ. Toward culture of single gametes: the
development of microfluidic platforms for assisted reproduction. Theriogenology.
2007 Sep 1;68 Suppl 1:S178-89. doi: 10.1016/j.theriogenology.2007.04.042. Epub
2007 May 23. PMID: 17524470.
213: Zheng T, Yu H, Alexander CM, Beebe DJ, Smith LM. Lectin-modified
microchannels for mammalian cell capture and purification. Biomed Microdevices.
2007 Aug;9(4):611-7. doi: 10.1007/s10544-007-9074-2. PMID: 17516171.
214: Abhyankar VV, Beebe DJ. Spatiotemporal micropatterning of cells on
arbitrary substrates. Anal Chem. 2007 Jun 1;79(11):4066-73. doi:
10.1021/ac062371p. Epub 2007 Apr 28. PMID: 17465529.
215: Yu H, Alexander CM, Beebe DJ. A plate reader-compatible microchannel array
for cell biology assays. Lab Chip. 2007 Mar;7(3):388-91. doi: 10.1039/b612358a.
Epub 2007 Jan 2. PMID: 17330172.
216: Warrick J, Meyvantsson I, Ju J, Beebe DJ. High-throughput microfluidics:
improved sample treatment and washing over standard wells. Lab Chip. 2007
Mar;7(3):316-21. doi: 10.1039/b613350a. Epub 2007 Jan 16. PMID: 17330162.
217: Agarwal AK, Dong L, Beebe DJ, Jiang H. Autonomously-triggered microfluidic
cooling using thermo-responsive hydrogels. Lab Chip. 2007 Mar;7(3):310-5. doi:
10.1039/b617767k. Epub 2007 Jan 3. PMID: 17330161.
218: Mohanty SK, Sohn LL, Beebe DJ. Hybrid polymer/thin film impedance system
for label free monitoring of cells. Conf Proc IEEE Eng Med Biol Soc.
2004;2004:2561-4. doi: 10.1109/IEMBS.2004.1403736. PMID: 17270796.
219: Kim D, Beebe DJ. Hydrogel-based reconfigurable components for microfluidic
devices. Lab Chip. 2007 Feb;7(2):193-8. doi: 10.1039/b612995a. Epub 2006 Dec 7.
PMID: 17268621.
220: Paguirigan A, Beebe DJ, Alexander CM. Simulating mouse mammary gland
development: cell ageing and its relation to stem and progenitor activity. Cell
Prolif. 2007 Feb;40(1):106-24. doi: 10.1111/j.1365-2184.2007.00416.x. PMID:
17227299; PMCID: PMC6760711.
221: Toepke MW, Beebe DJ. PDMS absorption of small molecules and consequences in
microfluidic applications. Lab Chip. 2006 Dec;6(12):1484-6. doi:
10.1039/b612140c. Epub 2006 Oct 4. PMID: 17203151.
222: Kaczmarek KA, Nammi K, Agarwal AK, Tyler ME, Haase SJ, Beebe DJ. Polarity
effect in electrovibration for tactile display. IEEE Trans Biomed Eng. 2006
Oct;53(10):2047-54. doi: 10.1109/TBME.2006.881804. PMID: 17019869; PMCID:
PMC2582732.
223: Mohanty SK, Kim D, Beebe DJ. Do-it-yourself microelectrophoresis chips with
integrated sample recovery. Electrophoresis. 2006 Oct;27(19):3772-8. doi:
10.1002/elps.200600238. PMID: 16960842.
224: Dong L, Agarwal AK, Beebe DJ, Jiang H. Adaptive liquid microlenses
activated by stimuli-responsive hydrogels. Nature. 2006 Aug 3;442(7102):551-4.
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225: Sridharamurthy SS, Agarwal AK, Beebe DJ, Jiang H. Dissolvable membranes as
sensing elements for microfluidics based biological/chemical sensors. Lab Chip.
2006 Jul;6(7):840-2. doi: 10.1039/b607066c. Epub 2006 May 31. PMID: 16804586.
226: Paguirigan A, Beebe DJ, Liu B, Alexander C. Mammary stem and progenitor
cells: tumour precursors? Eur J Cancer. 2006 Jun;42(9):1225-36. doi:
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227: Kim D, Chesler NC, Beebe DJ. A method for dynamic system characterization
using hydraulic series resistance. Lab Chip. 2006 May;6(5):639-44. doi:
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228: Atencia J, Beebe DJ. Capillary inserts in microcirculatory systems. Lab
Chip. 2006 Apr;6(4):575-7. doi: 10.1039/b514068d. Epub 2006 Jan 20. PMID:
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229: Atencia J, Beebe DJ. Steady flow generation in microcirculatory systems.
Lab Chip. 2006 Apr;6(4):567-74. doi: 10.1039/b514070f. Epub 2006 Jan 20. PMID:
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230: Tang H, Beebe DJ. An oral tactile interface for blind navigation. IEEE
Trans Neural Syst Rehabil Eng. 2006 Mar;14(1):116-23. doi:
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231: Paguirigan A, Beebe DJ. Gelatin based microfluidic devices for cell
culture. Lab Chip. 2006 Mar;6(3):407-13. doi: 10.1039/b517524k. Epub 2006 Jan
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232: Abhyankar VV, Lokuta MA, Huttenlocher A, Beebe DJ. Characterization of a
membrane-based gradient generator for use in cell-signaling studies. Lab Chip.
2006 Mar;6(3):389-93. doi: 10.1039/b514133h. Epub 2006 Feb 1. PMID: 16511622.
233: Wheeler MB, Rutledge JJ, Fischer-Brown A, VanEtten T, Malusky S, Beebe DJ.
Application of sexed semen technology to in vitro embryo production in cattle.
Theriogenology. 2006 Jan 7;65(1):219-27. doi:
10.1016/j.theriogenology.2005.09.032. Epub 2005 Nov 2. PMID: 16263159.
234: Clark SG, Haubert K, Beebe DJ, Ferguson CE, Wheeler MB. Reduction of
polyspermic penetration using biomimetic microfluidic technology during in vitro
fertilization. Lab Chip. 2005 Nov;5(11):1229-32. doi: 10.1039/b504397m. Epub
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235: Atencia J, Beebe DJ. Controlled microfluidic interfaces. Nature. 2005 Sep
29;437(7059):648-55. doi: 10.1038/nature04163. PMID: 16193039.
236: Yu H, Meyvantsson I, Shkel IA, Beebe DJ. Diffusion dependent cell behavior
in microenvironments. Lab Chip. 2005 Oct;5(10):1089-95. doi: 10.1039/b504403k.
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237: Eddington DT, Beebe DJ. Development of a disposable infusion system for the
delivery of protein therapeutics. Biomed Microdevices. 2005 Sep;7(3):223-30.
doi: 10.1007/s10544-005-3029-2. PMID: 16133810.
238: Sershen SR, Mensing GA, Ng M, Halas NJ, Beebe DJ, West JL. Independent
Optical Control of Microfluidic Valves Formed from Optomechanically Responsive
Nanocomposite Hydrogels. Adv Mater. 2005 Jun 6;17(11):1366-1368. doi:
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239: Jeong WJ, Kim JY, Choo J, Lee EK, Han CS, Beebe DJ, Seong GH, Lee SH.
Continuous fabrication of biocatalyst immobilized microparticles using
photopolymerization and immiscible liquids in microfluidic systems. Langmuir.
2005 Apr 26;21(9):3738-41. doi: 10.1021/la050105l. PMID: 15835930.
240: Zeringue HC, Wheeler MB, Beebe DJ. A microfluidic method for removal of the
zona pellucida from mammalian embryos. Lab Chip. 2005 Jan;5(1):108-10. doi:
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241: Zeringue HC, Rutledge JJ, Beebe DJ. Early mammalian embryo development
depends on cumulus removal technique. Lab Chip. 2005 Jan;5(1):86-90. doi:
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242: Atencia J, Beebe DJ. Magnetically-driven biomimetic micro pumping using
vortices. Lab Chip. 2004 Dec;4(6):598-602. doi: 10.1039/b407710e. Epub 2004 Nov
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243: Jeong W, Kim J, Kim S, Lee S, Mensing G, Beebe DJ. Hydrodynamic
microfabrication via”on the fly” photopolymerization of microscale fibers and
tubes. Lab Chip. 2004 Dec;4(6):576-80. doi: 10.1039/b411249k. Epub 2004 Nov 11.
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244: Mensing GA, Pearce TM, Graham MD, Beebe DJ. An externally driven magnetic
microstirrer. Philos Trans A Math Phys Eng Sci. 2004 May 15;362(1818):1059-68.
doi: 10.1098/rsta.2003.1362. PMID: 15306484.
245: Moorthy J, Mensing GA, Kim D, Mohanty S, Eddington DT, Tepp WH, Johnson EA,
Beebe DJ. Microfluidic tectonics platform: A colorimetric, disposable botulinum
toxin enzyme-linked immunosorbent assay system. Electrophoresis. 2004
Jun;25(10-11):1705-13. doi: 10.1002/elps.200405888. PMID: 15188260.
246: Raty S, Walters EM, Davis J, Zeringue H, Beebe DJ, Rodriguez-Zas SL,
Wheeler MB. Embryonic development in the mouse is enhanced via microchannel
culture. Lab Chip. 2004 Jun;4(3):186-90. doi: 10.1039/b316437c. Epub 2004 Mar
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247: Eddington DT, Liu RH, Moore JS, Beebe DJ. An organic self-regulating
microfluidic system. Lab Chip. 2001 Dec;1(2):96-9. doi: 10.1039/b108078d. Epub
2001 Nov 22. PMID: 15100866.
248: Khoury C, Mensing GA, Beebe DJ. Ultra rapid prototyping of microfluidic
systems using liquid phase photopolymerization. Lab Chip. 2002 Feb;2(1):50-5.
doi: 10.1039/b109344d. Epub 2002 Jan 10. PMID: 15100862.
249: Moorthy J, Beebe DJ. A hydrogel readout for autonomous detection of ions in
microchannels. Lab Chip. 2002 May;2(2):76-80. doi: 10.1039/b110094g. Epub 2002
Feb 26. PMID: 15100838.
250: Walker GM, Beebe DJ. An evaporation-based microfluidic sample concentration
method. Lab Chip. 2002 May;2(2):57-61. doi: 10.1039/b202473j. Epub 2002 Apr 30.
PMID: 15100834.
251: Walker G, Beebe DJ. A passive pumping method for microfluidic devices. Lab
Chip. 2002 Aug;2(3):131-4. doi: 10.1039/b204381e. Epub 2002 Aug 5. PMID:
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252: Moorthy J, Beebe DJ. In situ fabricated porous filters for microsystems.
Lab Chip. 2003 May;3(2):62-6. doi: 10.1039/b300450c. Epub 2003 Apr 30. PMID:
15100783.
253: Lee S, Jeong W, Beebe DJ. Microfluidic valve with cored glass microneedle
for microinjection. Lab Chip. 2003 Aug;3(3):164-7. doi: 10.1039/b305692a. Epub
2003 Jul 24. PMID: 15100768.
254: Walker GM, Zeringue HC, Beebe DJ. Microenvironment design considerations
for cellular scale studies. Lab Chip. 2004 Apr;4(2):91-7. doi: 10.1039/b311214d.
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255: Walters EM, Clark SG, Beebe DJ, Wheeler MB. Mammalian embryo culture in a
microfluidic device. Methods Mol Biol. 2004;254:375-82. doi:
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256: Zeringue HC, Beebe DJ. Microfluidic removal of cumulus cells from Mammalian
zygotes. Methods Mol Biol. 2004;254:365-74. doi: 10.1385/1-59259-741-6:365.
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257: Wheeler MB, Clark SG, Beebe DJ. Developments in in vitro technologies for
swine embryo production. Reprod Fertil Dev. 2004;16(1-2):15-25. doi:
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258: Eddington DT, Beebe DJ. Flow control with hydrogels. Adv Drug Deliv Rev.
2004 Feb 10;56(2):199-210. doi: 10.1016/j.addr.2003.08.013. PMID: 14741116.
259: Moorthy J, Beebe DJ. Organic and biomimetic designs for microfluidic
systems. Anal Chem. 2003 Jul 1;75(13):292A-301A. doi: 10.1021/ac0313567. PMID:
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260: Wang L, Beebe DJ. Characterization of a silicon-based shear-force sensor on
human subjects. IEEE Trans Biomed Eng. 2002 Nov;49(11):1340-7. doi:
10.1109/TBME.2002.804586. PMID: 12450364.
261: Zhao B, Moore JS, Beebe DJ. Principles of surface-directed liquid flow in
microfluidic channels. Anal Chem. 2002 Aug 15;74(16):4259-68. doi:
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262: Beebe DJ, Mensing GA, Walker GM. Physics and applications of microfluidics
in biology. Annu Rev Biomed Eng. 2002;4:261-86. doi:
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263: Hickman DL, Beebe DJ, Rodriguez-Zas SL, Wheeler MB. Comparison of static
and dynamic medium environments for culturing of pre-implantation mouse embryos.
Comp Med. 2002 Apr;52(2):122-6. PMID: 12022391.
264: Zhao B, Viernes NO, Moore JS, Beebe DJ. Control and applications of
immiscible liquids in microchannels. J Am Chem Soc. 2002 May 15;124(19):5284-5.
doi: 10.1021/ja025835j. PMID: 11996566.
265: Glasgow IK, Zeringue HC, Beebe DJ, Choi SJ, Lyman JT, Chan NG, Wheeler MB.
Handling individual mammalian embryos using microfluidics. IEEE Trans Biomed
Eng. 2001 May;48(5):570-8. doi: 10.1109/10.918596. PMID: 11341531.
266: Zhao B, Moore JS, Beebe DJ. Surface-directed liquid flow inside
microchannels. Science. 2001 Feb 9;291(5506):1023-6. doi:
10.1126/science.291.5506.1023. PMID: 11161212.
267: Beebe DJ, Moore JS, Yu Q, Liu RH, Kraft ML, Jo BH, Devadoss C. Microfluidic
tectonics: a comprehensive construction platform for microfluidic systems. Proc
Natl Acad Sci U S A. 2000 Dec 5;97(25):13488-93. doi: 10.1073/pnas.250273097.
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268: Folch A, Jo BH, Hurtado O, Beebe DJ, Toner M. Microfabricated elastomeric
stencils for micropatterning cell cultures. J Biomed Mater Res. 2000
Nov;52(2):346-53. doi: 10.1002/1097-4636(200011)52:2<346::aid-jbm14>3.0.co;2-h.
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269: Beebe DJ, Moore JS, Bauer JM, Yu Q, Liu RH, Devadoss C, Jo BH. Functional
hydrogel structures for autonomous flow control inside microfluidic channels.
Nature. 2000 Apr 6;404(6778):588-90. doi: 10.1038/35007047. PMID: 10766238.
270: Trumbull JD, Glasgow IK, Beebe DJ, Magin RL. Integrating microfabricated
fluidic systems and NMR spectroscopy. IEEE Trans Biomed Eng. 2000 Jan;47(1):3-7.
doi: 10.1109/10.817611. PMID: 10646271.
271: Tang H, Beebe DJ. A microfabricated electrostatic haptic display for
persons with visual impairments. IEEE Trans Rehabil Eng. 1998 Sep;6(3):241-8.
doi: 10.1109/86.712216. PMID: 9749901.
272: Beebe DJ, Denton DD, Radwin RG, Webster JG. A silicon-based tactile sensor
for finger-mounted applications. IEEE Trans Biomed Eng. 1998 Feb;45(2):151-9.
doi: 10.1109/10.661263. PMID: 9473838.
273: Wang L, Beebe DJ, Williams AR, Easley KD. Electrothermal branding for
embryo labeling. IEEE Trans Biomed Eng. 1997 Nov;44(11):1128-38. doi:
10.1109/10.641341. PMID: 9353993.